Acute Myeloid Leukemia Research Today is a free monthly online journal that collates and summarizes the latest research about Acute Myeloid Leukemia, including details on aml, symptoms, treatment, information.

Improved detection of minimal acute myeloid leukemia cells by the use of the combined parameters of XE-2100 hematology analyzer.

Tohyama K, Shiga S, Itose Y, Uchihashi K, Ohkura M, Takahashi K, Itoh M, Ichiyama S, Hamaguchi Y

Department of Clinical Pathology and Laboratory Medicine, Kawasaki Medical School, Okayama, Japan. ktohyama@med.kawasaki-m.ac.jp

BACKGROUND: For the diagnosis and therapy of acute leukemia, it is important to detect a small number of leukemic cells precisely. Although several automated hematology analyzers that carry blast-detecting programs have been developed, they do not exert sufficient detection sensitivity to exceed the sensitivity of manual eye counting method. METHODS: We constructed a new blast-detecting program by combining the numerical information acquired from five cytometric parameters presented by XE-2100. The sensitivity and specificity of this blast multi-scoring program were assessed in comparison with the Blasts flag program equipped originally in XE-2100. RESULTS: The blast-detecting sensitivity was found to be highly improved in the blast multi-scoring program as compared with the Blasts flag program without much decreasing the specificity. A small number of leukemic myeloblasts was detected at the better sensitivity than the eye counting method in the clinical course of the patients with acute myeloid leukemia. CONCLUSIONS: The daily practical use of this blast multi-scoring program will surely contribute to sensitive, objective, and real-time evaluation of the control of acute myeloid leukemia with a low cost.

Published 20 June 2005 in Cytometry B Clin Cytom, 66(1): 18-24.
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