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Immunophenotyping of surface antigens in acute myeloid leukemia by flow cytometry after red blood cell lysis.

Schwonzen M, Diehl V, Dellanna M, Staib P

St. Walburga-Hospital, D-59870 Meschede, Germany. schwonzen@freenet.de

Immunophenotyping of acute leukemia using flow cytometry after density gradient separation (dg-sep) of mononuclear cells is the international gold standard. But destroying red cells by whole blood lysis (wb-lysis) after direct staining has found a broad usage. Both methods revealed congruent results in phenotyping of 26 cases of acute myeloid leukemias by testing CD4, CD7, CD11b, CD11c, CD13, CD14, CD15, CD33, CD34, CD65s, Glycophorin A and HLA-DR antigens. Mean values of these tested antigens were very similar. Only low antigen expression of CD2 was missed in the phenotype of two AML cases by the wb-lysis (23 and 29% positive cells by the dg-sep).

Published 19 November 2006 in Leuk Res, 31(1): 113-6.
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